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Procell Inc human crc cell line sw480
Human Crc Cell Line Sw480, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crc+cell+line+sw480/pm42271166-57-1-12?v=Procell+Inc
Average 86 stars, based on 1 article reviews

human crc cell line sw480 - by Bioz Stars, 2026-06

86/100 stars

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A hypoxia-characteristic cluster identified by Microwell-seq exhibited pronounced ferroptosis resistance in MSS CRC cells. (A and B) t-distributed stochastic neighbor embedding (t-SNE) plot of Microwell-seq analysis based on gene expressions of SW480 and WiDr. (C and D) Comparative gene set enrichment analysis (GSEA) of signaling pathways in different clusters. The red color represents up-regulation and blue represents down-regulation, calculated with the formula: ± Log2|NES/p.adjust|. Grey color represents no enrichment in the indicated pathway. NES: normalized enrichment score. (E and F) GSEA analysis showed the indicated pathway activity between the hypoxia cluster and other clusters. (G and H) Correlation analysis of hypoxia scores, glycolysis scores, and ferroptosis suppressor scores in WiDr and SW480 cells was performed using Pearson's method. (I and J) The ferroptosis suppressor score of SW480 and WiDr with DMSO or RSL3 treatment was analyzed by AddModuleScore tool. P values were calculated by Wilcox.test. (K and L) The ferroptosis suppressor score of hypoxia cluster in SW480 and WiDr treated with DMSO or RSL3 was shown. P values were calculated by Wilcox.test.

Journal: Redox Biology

Article Title: Targeting HIF-1α promotes ferroptosis and boosts antitumor immunity in MSS colorectal cancer

doi: 10.1016/j.redox.2026.104151

Figure Lengend Snippet: A hypoxia-characteristic cluster identified by Microwell-seq exhibited pronounced ferroptosis resistance in MSS CRC cells. (A and B) t-distributed stochastic neighbor embedding (t-SNE) plot of Microwell-seq analysis based on gene expressions of SW480 and WiDr. (C and D) Comparative gene set enrichment analysis (GSEA) of signaling pathways in different clusters. The red color represents up-regulation and blue represents down-regulation, calculated with the formula: ± Log2|NES/p.adjust|. Grey color represents no enrichment in the indicated pathway. NES: normalized enrichment score. (E and F) GSEA analysis showed the indicated pathway activity between the hypoxia cluster and other clusters. (G and H) Correlation analysis of hypoxia scores, glycolysis scores, and ferroptosis suppressor scores in WiDr and SW480 cells was performed using Pearson's method. (I and J) The ferroptosis suppressor score of SW480 and WiDr with DMSO or RSL3 treatment was analyzed by AddModuleScore tool. P values were calculated by Wilcox.test. (K and L) The ferroptosis suppressor score of hypoxia cluster in SW480 and WiDr treated with DMSO or RSL3 was shown. P values were calculated by Wilcox.test.

Article Snippet: The human MSS CRC cell lines SW480, HT-29, and WiDr, human HEK293T, and mouse MSS CRC cell line CT26 were obtained from American Type Culture Collection (ATCC).

Techniques: Protein-Protein interactions, Activity Assay

HIF-1α nuclear distribution increased in RSL3-resistant CT26 cells and significantly promoted tumourigenicity and metastasis. (A) The diagram demonstrated the procedure for sphere formation. (B and C) The representative images of sphere formation in MSS CRC cells and quantification analysis of sphere number derived from SW480, HT-29, and WiDr. (D) HIF-1α expression in WiDr was detected by western blotting. (E) qPCR analyzed the indicated gene expression involved in glycolysis in WiDr spheres. (F) Cell viability of parental CT26 and RSL3-resistant CT26 (Re-CT26). (G) Cytoplasm and nuclear HIF-1α expression in CT26. α-tubulin was used as an internal reference for the cytoplasm, and Histone 3 was used as a nuclear reference. (H) qPCR analyzed the indicated gene expression involved in glycolysis in CT26. (I) Image of subcutaneous tumors derived from parental CT26 and Re-CT26. (J) Tumor volume determined by formula: 0.52 × Long × width 2 . (K)Tumor weight of subcutaneous tumors. (L) Bioluminescent images in liver metastatic models. (M) Images of liver metastasis derived from parental CT26 and Re-CT26. (N) Number of liver nodules in liver metastasis models. (O) Representative hematoxylin and eosin (H&E) and immunohistochemical staining images from liver metastasis models. Scale bar: 25 μm. (P) Quantification of immunohistochemical staining results shown in (O). Data are shown as means ± SD. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ns: not significant. Two-way ANOVA in (J), others unpaired two-tailed Student's t -test.

Journal: Redox Biology

Article Title: Targeting HIF-1α promotes ferroptosis and boosts antitumor immunity in MSS colorectal cancer

doi: 10.1016/j.redox.2026.104151

Figure Lengend Snippet: HIF-1α nuclear distribution increased in RSL3-resistant CT26 cells and significantly promoted tumourigenicity and metastasis. (A) The diagram demonstrated the procedure for sphere formation. (B and C) The representative images of sphere formation in MSS CRC cells and quantification analysis of sphere number derived from SW480, HT-29, and WiDr. (D) HIF-1α expression in WiDr was detected by western blotting. (E) qPCR analyzed the indicated gene expression involved in glycolysis in WiDr spheres. (F) Cell viability of parental CT26 and RSL3-resistant CT26 (Re-CT26). (G) Cytoplasm and nuclear HIF-1α expression in CT26. α-tubulin was used as an internal reference for the cytoplasm, and Histone 3 was used as a nuclear reference. (H) qPCR analyzed the indicated gene expression involved in glycolysis in CT26. (I) Image of subcutaneous tumors derived from parental CT26 and Re-CT26. (J) Tumor volume determined by formula: 0.52 × Long × width 2 . (K)Tumor weight of subcutaneous tumors. (L) Bioluminescent images in liver metastatic models. (M) Images of liver metastasis derived from parental CT26 and Re-CT26. (N) Number of liver nodules in liver metastasis models. (O) Representative hematoxylin and eosin (H&E) and immunohistochemical staining images from liver metastasis models. Scale bar: 25 μm. (P) Quantification of immunohistochemical staining results shown in (O). Data are shown as means ± SD. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ns: not significant. Two-way ANOVA in (J), others unpaired two-tailed Student's t -test.

Article Snippet: The human MSS CRC cell lines SW480, HT-29, and WiDr, human HEK293T, and mouse MSS CRC cell line CT26 were obtained from American Type Culture Collection (ATCC).

Techniques: Derivative Assay, Expressing, Western Blot, Gene Expression, Immunohistochemical staining, Staining, Two Tailed Test

P4HA1 was a major factor regulated by HIF-1α and was enriched in ferroptosis-resistant cells. (A) Venn diagram screening 10 genes commonly induced by hypoxia and the glycolysis pathway in SW480 and WiDr by single-cell sequencing. (B) Gene expression heatmap showed the distribution of genes in different clusters in the presence or absence of RSL3. D: DMSO, R: RSL3. (C) Correlation between ferroptosis suppressor score and P4HA1 in MSS CRC containing 119 patients using Pearson's method. (D) Kaplan-Meier plots of RFS in MSS colon cancer patients according to P4HA1 expression. (E) The correlation between HIF-1α and P4HA1 was evaluated by Spearman's analysis in a colon adenocarcinoma cohort of 457 patients. (F) Relative P4HA1 mRNA expression after HIF-1α knockdown, detected by qPCR. (G) Relative P4HA1 protein expression after HIF-1α knockdown, detected by Western blot. (H) Relative P4HA1 mRNA expression after HIF-1α overexpression, detected by qPCR. (I) Relative P4HA1 protein expression after HIF-1α overexpression, detected by Western blot. (J) Relative P4HA1 expression, detected by qPCR. (K) HIF-1α binding motif predicted from JASPAR. (L) The prospective binding site of HIF-1α on the promoter of P4HA1. (M) ChIP assay of HIF-1α and IgG in parental CT26 cells or Re-CT26 cells, followed by qPCR for the binding sequences.

Journal: Redox Biology

Article Title: Targeting HIF-1α promotes ferroptosis and boosts antitumor immunity in MSS colorectal cancer

doi: 10.1016/j.redox.2026.104151

Figure Lengend Snippet: P4HA1 was a major factor regulated by HIF-1α and was enriched in ferroptosis-resistant cells. (A) Venn diagram screening 10 genes commonly induced by hypoxia and the glycolysis pathway in SW480 and WiDr by single-cell sequencing. (B) Gene expression heatmap showed the distribution of genes in different clusters in the presence or absence of RSL3. D: DMSO, R: RSL3. (C) Correlation between ferroptosis suppressor score and P4HA1 in MSS CRC containing 119 patients using Pearson's method. (D) Kaplan-Meier plots of RFS in MSS colon cancer patients according to P4HA1 expression. (E) The correlation between HIF-1α and P4HA1 was evaluated by Spearman's analysis in a colon adenocarcinoma cohort of 457 patients. (F) Relative P4HA1 mRNA expression after HIF-1α knockdown, detected by qPCR. (G) Relative P4HA1 protein expression after HIF-1α knockdown, detected by Western blot. (H) Relative P4HA1 mRNA expression after HIF-1α overexpression, detected by qPCR. (I) Relative P4HA1 protein expression after HIF-1α overexpression, detected by Western blot. (J) Relative P4HA1 expression, detected by qPCR. (K) HIF-1α binding motif predicted from JASPAR. (L) The prospective binding site of HIF-1α on the promoter of P4HA1. (M) ChIP assay of HIF-1α and IgG in parental CT26 cells or Re-CT26 cells, followed by qPCR for the binding sequences.

Article Snippet: The human MSS CRC cell lines SW480, HT-29, and WiDr, human HEK293T, and mouse MSS CRC cell line CT26 were obtained from American Type Culture Collection (ATCC).

Techniques: Single Cell, Sequencing, Gene Expression, Expressing, Knockdown, Western Blot, Over Expression, Binding Assay

HIF-1α inhibition enhanced ferroptosis inducer sensitivity in MSS CRC cells. (A and B) Cell viability of HT-29 or SW480 treated with RSL3, BAY 87-2243 alone or in combination treatment. (C and D) DCFH-DA oxidation in HT-29 or SW480 treated with RSL3, BAY 87-2243 alone or in combination treatment were quantified using flow cytometry with DCFH-DA probe. (E and F) Lipid peroxidation levels of HT-29 or SW480 treated with RSL3, BAY 87-2243 alone or in combination treatment were detected using flow cytometry with C11-BODIPY 581/591 (FITC channel; excitation/emission: 488/510 nm). (G) MDA levels in cells subjected to the indicated treatment. (H) GSH levels in cells subjected to the indicated treatment. Results are shown as means ± SD. ∗ P < 0.05 ; ∗∗ P < 0.01 ; ∗∗∗ P < 0.001 ; ∗∗∗∗ P < 0.0001 . P values were calculated by one-way ANOVA.

Journal: Redox Biology

Article Title: Targeting HIF-1α promotes ferroptosis and boosts antitumor immunity in MSS colorectal cancer

doi: 10.1016/j.redox.2026.104151

Figure Lengend Snippet: HIF-1α inhibition enhanced ferroptosis inducer sensitivity in MSS CRC cells. (A and B) Cell viability of HT-29 or SW480 treated with RSL3, BAY 87-2243 alone or in combination treatment. (C and D) DCFH-DA oxidation in HT-29 or SW480 treated with RSL3, BAY 87-2243 alone or in combination treatment were quantified using flow cytometry with DCFH-DA probe. (E and F) Lipid peroxidation levels of HT-29 or SW480 treated with RSL3, BAY 87-2243 alone or in combination treatment were detected using flow cytometry with C11-BODIPY 581/591 (FITC channel; excitation/emission: 488/510 nm). (G) MDA levels in cells subjected to the indicated treatment. (H) GSH levels in cells subjected to the indicated treatment. Results are shown as means ± SD. ∗ P < 0.05 ; ∗∗ P < 0.01 ; ∗∗∗ P < 0.001 ; ∗∗∗∗ P < 0.0001 . P values were calculated by one-way ANOVA.

Article Snippet: The human MSS CRC cell lines SW480, HT-29, and WiDr, human HEK293T, and mouse MSS CRC cell line CT26 were obtained from American Type Culture Collection (ATCC).

Techniques: Inhibition, Flow Cytometry

Isolation, characterization and cellular internalization of PELNs. (A) Schematic diagram of the preparation process for isolating PELNs from the peel of Punica granatum. (B) Representative transmission electron microscopy image of PELNs. Scale bar, 200 nm (left); 100 nm (right). (C) PELN concentration and size distribution. (D) Zeta potential distribution of PELNs. (E) PKH26-labeled PELNs (red) were received by SW480 cells [nuclei stained by DAPI (blue)]. Scale bar, 25 µm. PELNs, pomegranate peel-derived exosome-like nanoparticles; UC, ultracentrifugation; PBS, phosphate-buffered saline.

Journal: Oncology Letters

Article Title: Pomegranate peel-derived exosome-like nanoparticles: A discarded treasure trove for colorectal cancer treatment

doi: 10.3892/ol.2026.15546

Figure Lengend Snippet: Isolation, characterization and cellular internalization of PELNs. (A) Schematic diagram of the preparation process for isolating PELNs from the peel of Punica granatum. (B) Representative transmission electron microscopy image of PELNs. Scale bar, 200 nm (left); 100 nm (right). (C) PELN concentration and size distribution. (D) Zeta potential distribution of PELNs. (E) PKH26-labeled PELNs (red) were received by SW480 cells [nuclei stained by DAPI (blue)]. Scale bar, 25 µm. PELNs, pomegranate peel-derived exosome-like nanoparticles; UC, ultracentrifugation; PBS, phosphate-buffered saline.

Article Snippet: The CRC cell line SW480 utilized in the present study was obtained from Procell Life Science & Technology Co., Ltd. SW480 cells were cultured in Dulbecco's Modified Eagle medium (DMEM; Invitrogen; Thermo Fisher Scientific, Inc.) with 10% fetal bovine serum (FBS; HyCloneTM; Cytiva) and 1% penicillin/streptomycin (PS) solution in a 37°C cell culture incubator with 5% CO 2 .

Techniques: Isolation, Transmission Assay, Electron Microscopy, Concentration Assay, Zeta Potential Analyzer, Labeling, Staining, Derivative Assay, Saline

Proliferation and apoptosis of SW480 cells after treatment with PELNs. (A and B) Schematic and quantitative diagrams of proliferation of SW480 cells treated with PBS or PELNs. Data are presented as the mean ± SD (n=5). (C and D) Schematic and quantitative diagrams of clone formation of SW480 cells treated with PBS or PELNs. Data are presented as the mean ± SD (n=3). (E and F) Schematic and quantitative diagrams of flow cytometric analysis of SW480 cell apoptosis treated with PBS or PELNs. Data are presented as the mean ± SD (n=3). **P<0.01 and ***P<0.001. PELNs, pomegranate peel-derived exosome-like nanoparticles; PBS, phosphate-buffered saline; ns, not significant.

Journal: Oncology Letters

Article Title: Pomegranate peel-derived exosome-like nanoparticles: A discarded treasure trove for colorectal cancer treatment

doi: 10.3892/ol.2026.15546

Figure Lengend Snippet: Proliferation and apoptosis of SW480 cells after treatment with PELNs. (A and B) Schematic and quantitative diagrams of proliferation of SW480 cells treated with PBS or PELNs. Data are presented as the mean ± SD (n=5). (C and D) Schematic and quantitative diagrams of clone formation of SW480 cells treated with PBS or PELNs. Data are presented as the mean ± SD (n=3). (E and F) Schematic and quantitative diagrams of flow cytometric analysis of SW480 cell apoptosis treated with PBS or PELNs. Data are presented as the mean ± SD (n=3). **P<0.01 and ***P<0.001. PELNs, pomegranate peel-derived exosome-like nanoparticles; PBS, phosphate-buffered saline; ns, not significant.

Techniques: Derivative Assay, Saline

SW480 cell migration after treatment with PELNs. (A and B) Schematic and quantitative diagrams of wound healing of SW480 cells treated with PBS or PELNs. (C and D) Schematic and quantitative diagrams of Transwell migration of SW480 cells treated with PBS or PELNs. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001. PELNs, pomegranate peel-derived exosome-like nanoparticles; PBS, phosphate-buffered saline; ns, not significant.

Journal: Oncology Letters

Article Title: Pomegranate peel-derived exosome-like nanoparticles: A discarded treasure trove for colorectal cancer treatment

doi: 10.3892/ol.2026.15546

Figure Lengend Snippet: SW480 cell migration after treatment with PELNs. (A and B) Schematic and quantitative diagrams of wound healing of SW480 cells treated with PBS or PELNs. (C and D) Schematic and quantitative diagrams of Transwell migration of SW480 cells treated with PBS or PELNs. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001. PELNs, pomegranate peel-derived exosome-like nanoparticles; PBS, phosphate-buffered saline; ns, not significant.

Techniques: Migration, Derivative Assay, Saline

Anti-colorectal cancer molecular mechanism of PELNs. (A) Sample correlation analysis heat map. (B and C) Volcano plot map and cluster analysis diagram of differentially expressed genes in SW480 cells treated with phosphate-buffered saline or PELNs. (D and E) Balloon plots of KEGG pathway (top 20) and GO term enrichment (top 20) analyses. PELNs, pomegranate peel-derived exosome-like nanoparticles; KEGG, Kyoto Encyclopedia of Genes and Genomes; GO, Gene Ontology.

Journal: Oncology Letters

Article Title: Pomegranate peel-derived exosome-like nanoparticles: A discarded treasure trove for colorectal cancer treatment

doi: 10.3892/ol.2026.15546

Figure Lengend Snippet: Anti-colorectal cancer molecular mechanism of PELNs. (A) Sample correlation analysis heat map. (B and C) Volcano plot map and cluster analysis diagram of differentially expressed genes in SW480 cells treated with phosphate-buffered saline or PELNs. (D and E) Balloon plots of KEGG pathway (top 20) and GO term enrichment (top 20) analyses. PELNs, pomegranate peel-derived exosome-like nanoparticles; KEGG, Kyoto Encyclopedia of Genes and Genomes; GO, Gene Ontology.

Techniques: Saline, Derivative Assay

AL445238.2 regulates the survival of colon cancer cells. (A) Validation of gene manipulation efficiency in SW480 and DLD1 cell lines with stable overexpression and knockdown of AL445238.2 . (B) Cell Counting Kit-8 assay of SW480 and DLD1 stable cell lines. (C) Proportion of apoptotic SW480 and DLD1 stable cell lines (percentage of supernatant LDH/total LDH). (D) Statistical analysis of the Annexin V and PI staining assay(Proportion percentage of Apoptotic cells). (E) Annexin V and PI staining of SW480 and DLD1 stable cell lines. ** P<0.01, *** P<0.001, **** P<0.0001. NC, negative control; sh, short hairpin; OE, overexpression; LDH, lactate dehydrogenase; ns, not significant.

Journal: International Journal of Oncology

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer

doi: 10.3892/ijo.2026.5858

Figure Lengend Snippet: AL445238.2 regulates the survival of colon cancer cells. (A) Validation of gene manipulation efficiency in SW480 and DLD1 cell lines with stable overexpression and knockdown of AL445238.2 . (B) Cell Counting Kit-8 assay of SW480 and DLD1 stable cell lines. (C) Proportion of apoptotic SW480 and DLD1 stable cell lines (percentage of supernatant LDH/total LDH). (D) Statistical analysis of the Annexin V and PI staining assay(Proportion percentage of Apoptotic cells). (E) Annexin V and PI staining of SW480 and DLD1 stable cell lines. ** P<0.01, *** P<0.001, **** P<0.0001. NC, negative control; sh, short hairpin; OE, overexpression; LDH, lactate dehydrogenase; ns, not significant.

Article Snippet: The CRC cell lines SW480 and DLD1 were purchased from the American Type Culture Collection.

Techniques: Biomarker Discovery, Over Expression, Knockdown, Cell Counting, Stable Transfection, Staining, Negative Control

AL445238.2 promotes the stemness and mitochondrial stability of colon cancer cells. (A) The expression of CD133 in SW480 and DLD1 stable cell lines was detected with flow cytometry. (B) The statistical analysis of CD133 MFI. (C) TMRM staining of SW480 and DLD1 stable cell lines. (D) The statistical analysis of TMRM MFI. (E) Tumor spheroid formation assay of SW480 and DLD1 stable cell lines. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. MFI, mean fluorescence intensity; TMRM, tetramethylrhodamine; NC, negative control; sh, short hairpin; OE, overexpression.

Journal: International Journal of Oncology

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer

doi: 10.3892/ijo.2026.5858

Figure Lengend Snippet: AL445238.2 promotes the stemness and mitochondrial stability of colon cancer cells. (A) The expression of CD133 in SW480 and DLD1 stable cell lines was detected with flow cytometry. (B) The statistical analysis of CD133 MFI. (C) TMRM staining of SW480 and DLD1 stable cell lines. (D) The statistical analysis of TMRM MFI. (E) Tumor spheroid formation assay of SW480 and DLD1 stable cell lines. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. MFI, mean fluorescence intensity; TMRM, tetramethylrhodamine; NC, negative control; sh, short hairpin; OE, overexpression.

Article Snippet: The CRC cell lines SW480 and DLD1 were purchased from the American Type Culture Collection.

Techniques: Expressing, Stable Transfection, Flow Cytometry, Staining, Tube Formation Assay, Fluorescence, Negative Control, Over Expression

AL445238.2 binding to USP4 stabilizes Bcl2 to regulate colon cancer cell apoptosis and stemness. (A) Pulldown assay with AL445238.2 and western blot detection of USP4. (B) Gene colocalization was detected by immunofluorescence (magnification, ×200). (C) Validation of gene manipulation efficiency in SW480 and DLD1 cell lines with stable overexpression and knockdown of USP4. (D) Western blotting was used to evaluate the expression of BAX, Bcl2 and C-CASP3 in stable SW480 cells; BAX and Bcl2 were normalized against GAPDH, C-CASP3 was normalized against total CASP3. Western blotting was used to evaluate the expression of ALDH1A1, Bmi1 and EpCAM in stable (E) DLD1 and (F) SW480 cells. (G) Co-immunoprecipitation experiments were used to verify the interaction between USP4 and Bcl2. (H) Western blotting was used to evaluate the effects of USP4 knockdown and MG132 on Bcl2 protein expression. (I) Protein half-life experiments were used to evaluate the effects of USP4 and AL445238.2 on the stability of Bcl2 protein. ** P<0.01, *** P<0.001, **** P<0.0001. USP4, ubiquitin specific peptidase 4; RIP, RNA immunoprecipitation; Ctrl, control; ALDH1A1, aldehyde dehydrogenase 1 family member A1; Bmi1, B lymphoma Mo-MLV insertion region 1; EpCAM, epithelial cell adhesion molecule; CHX, cycloheximide; OE, overexpression; sh, short hairpin; NC, negative control; ns, not significant; C-CASP3, cleaved caspase-3.

Journal: International Journal of Oncology

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer

doi: 10.3892/ijo.2026.5858

Figure Lengend Snippet: AL445238.2 binding to USP4 stabilizes Bcl2 to regulate colon cancer cell apoptosis and stemness. (A) Pulldown assay with AL445238.2 and western blot detection of USP4. (B) Gene colocalization was detected by immunofluorescence (magnification, ×200). (C) Validation of gene manipulation efficiency in SW480 and DLD1 cell lines with stable overexpression and knockdown of USP4. (D) Western blotting was used to evaluate the expression of BAX, Bcl2 and C-CASP3 in stable SW480 cells; BAX and Bcl2 were normalized against GAPDH, C-CASP3 was normalized against total CASP3. Western blotting was used to evaluate the expression of ALDH1A1, Bmi1 and EpCAM in stable (E) DLD1 and (F) SW480 cells. (G) Co-immunoprecipitation experiments were used to verify the interaction between USP4 and Bcl2. (H) Western blotting was used to evaluate the effects of USP4 knockdown and MG132 on Bcl2 protein expression. (I) Protein half-life experiments were used to evaluate the effects of USP4 and AL445238.2 on the stability of Bcl2 protein. ** P<0.01, *** P<0.001, **** P<0.0001. USP4, ubiquitin specific peptidase 4; RIP, RNA immunoprecipitation; Ctrl, control; ALDH1A1, aldehyde dehydrogenase 1 family member A1; Bmi1, B lymphoma Mo-MLV insertion region 1; EpCAM, epithelial cell adhesion molecule; CHX, cycloheximide; OE, overexpression; sh, short hairpin; NC, negative control; ns, not significant; C-CASP3, cleaved caspase-3.

Article Snippet: The CRC cell lines SW480 and DLD1 were purchased from the American Type Culture Collection.

Techniques: Binding Assay, Western Blot, Immunofluorescence, Biomarker Discovery, Over Expression, Knockdown, Expressing, Immunoprecipitation, Ubiquitin Proteomics, RNA Immunoprecipitation, Control, Negative Control

USP4 regulates the survival of colon cancer cells. (A) Cell Counting Kit-8 Assay of SW480 and DLD1 stable cell lines. (B) Proportion of apoptotic SW480 and DLD1 stable cell lines (percentage of supernatant LDH/total LDH). (C) Statistical analysis of the Annexin V and PI staining assay (percentage of apoptotic cells). (D) Annexin V and PI staining of SW480 and DLD1 stable cell lines. ** P<0.01, *** P<0.001, **** P<0.0001. USP4, ubiquitin specific peptidase 4; OE, overexpression; sh, short hairpin; NC, negative control; ns, not significant; LDH, lactate dehydrogenase.

Journal: International Journal of Oncology

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer

doi: 10.3892/ijo.2026.5858

Figure Lengend Snippet: USP4 regulates the survival of colon cancer cells. (A) Cell Counting Kit-8 Assay of SW480 and DLD1 stable cell lines. (B) Proportion of apoptotic SW480 and DLD1 stable cell lines (percentage of supernatant LDH/total LDH). (C) Statistical analysis of the Annexin V and PI staining assay (percentage of apoptotic cells). (D) Annexin V and PI staining of SW480 and DLD1 stable cell lines. ** P<0.01, *** P<0.001, **** P<0.0001. USP4, ubiquitin specific peptidase 4; OE, overexpression; sh, short hairpin; NC, negative control; ns, not significant; LDH, lactate dehydrogenase.

Article Snippet: The CRC cell lines SW480 and DLD1 were purchased from the American Type Culture Collection.

Techniques: Cell Counting, Stable Transfection, Staining, Ubiquitin Proteomics, Over Expression, Negative Control

USP4 promotes stemness and mitochondrial stability of colon cancer cells. (A) Western blotting was used to evaluate the expression of BAX, Bcl2 and C-CASP3 in stable SW480 cells; BAX and Bcl2 were normalized against GAPDH, C-CASP3 was normalized against total CASP3. (B) The expression of CD133 in the SW480 and DLD1 stable cell lines was detected with flow cytometry. (C) Statistical analysis of the CD133 MFI. (D) Statistical analysis of the TMRM MFI. (E) TMRM staining of SW480 and DLD1 stable cell lines. (F) Tumor spheroid formation assay of SW480 and DLD1 stable cell lines. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. MFI, mean fluorescence intensity; TMRM, tetramethylrhodamine; C-CASP3, cleaved caspase-3; NC, negative control; USP4, ubiquitin specific peptidase 4; sh, short hairpin; OE, overexpression.

Journal: International Journal of Oncology

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer

doi: 10.3892/ijo.2026.5858

Figure Lengend Snippet: USP4 promotes stemness and mitochondrial stability of colon cancer cells. (A) Western blotting was used to evaluate the expression of BAX, Bcl2 and C-CASP3 in stable SW480 cells; BAX and Bcl2 were normalized against GAPDH, C-CASP3 was normalized against total CASP3. (B) The expression of CD133 in the SW480 and DLD1 stable cell lines was detected with flow cytometry. (C) Statistical analysis of the CD133 MFI. (D) Statistical analysis of the TMRM MFI. (E) TMRM staining of SW480 and DLD1 stable cell lines. (F) Tumor spheroid formation assay of SW480 and DLD1 stable cell lines. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. MFI, mean fluorescence intensity; TMRM, tetramethylrhodamine; C-CASP3, cleaved caspase-3; NC, negative control; USP4, ubiquitin specific peptidase 4; sh, short hairpin; OE, overexpression.

Article Snippet: The CRC cell lines SW480 and DLD1 were purchased from the American Type Culture Collection.

Techniques: Western Blot, Expressing, Stable Transfection, Flow Cytometry, Staining, Tube Formation Assay, Fluorescence, Negative Control, Ubiquitin Proteomics, Over Expression

AL445238.2 controlled USP4 promotes colon cancer cell migration. Migration assay of DLD1 and SW480 cells treated with shNC, sh- AL445238.2 , Vector or OE- AL445238.2 . (A) Representative images show migrated cells stained with crystal violet. (B) Quantitative analysis of migrated cell numbers in DLD1 (upper panel) and SW480 (lower panel) cells following AL445238.2 knockdown or overexpression. Migration assay of DLD1 and SW480 cells treated with shNC, sh-USP4, Vector or OE-USP4. (C) Representative images show migrated cells stained with crystal violet. (D) Quantitative analysis of migrated cell numbers in DLD1 (upper panel) and SW480 (lower panel) cells following USP4 knockdown or overexpression. Migration assay of DLD1 and SW480 cells with combined treatments: NC, OE- AL445238.2 , OE- AL445238.2 + OE-USP4 or OE- AL445238.2 + sh-USP4. (E) Representative images show migrated cells. (F) Quantitative analysis of migrated cell numbers in DLD1 (left panel) and SW480 (right panel) cells under combined treatment conditions. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. sh, short hairpin; NC, negative control; USP4, ubiquitin specific peptidase 4; OE, overexpression.

Journal: International Journal of Oncology

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer

doi: 10.3892/ijo.2026.5858

Figure Lengend Snippet: AL445238.2 controlled USP4 promotes colon cancer cell migration. Migration assay of DLD1 and SW480 cells treated with shNC, sh- AL445238.2 , Vector or OE- AL445238.2 . (A) Representative images show migrated cells stained with crystal violet. (B) Quantitative analysis of migrated cell numbers in DLD1 (upper panel) and SW480 (lower panel) cells following AL445238.2 knockdown or overexpression. Migration assay of DLD1 and SW480 cells treated with shNC, sh-USP4, Vector or OE-USP4. (C) Representative images show migrated cells stained with crystal violet. (D) Quantitative analysis of migrated cell numbers in DLD1 (upper panel) and SW480 (lower panel) cells following USP4 knockdown or overexpression. Migration assay of DLD1 and SW480 cells with combined treatments: NC, OE- AL445238.2 , OE- AL445238.2 + OE-USP4 or OE- AL445238.2 + sh-USP4. (E) Representative images show migrated cells. (F) Quantitative analysis of migrated cell numbers in DLD1 (left panel) and SW480 (right panel) cells under combined treatment conditions. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. sh, short hairpin; NC, negative control; USP4, ubiquitin specific peptidase 4; OE, overexpression.

Article Snippet: The CRC cell lines SW480 and DLD1 were purchased from the American Type Culture Collection.

Techniques: Migration, Plasmid Preparation, Staining, Knockdown, Over Expression, Negative Control, Ubiquitin Proteomics

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